Journal: Journal of Virology

Article Title: Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I

doi: 10.1128/jvi.01134-06

Figure Lengend Snippet: FIG. 2. Anti-SR-BI antibodies inhibit JFH-1 NS5A protein expression. Indirect immunofluorescence analysis of NS5A protein expressed in JFH-1-infected Huh-7 cells alone (A) or in the presence of anti-CD81 (25 g/ml) (D), anti-SR-BI (1:50) (E), or a combination of both anti-CD81 and anti-SR-BI antibodies (F). Huh-7 cells were incubated with equivalent concentrations of isotype-matched mouse IgG (mIgG) (B) and PI rat serum (C) as controls for the anti-CD81 and anti-SR-BI antibodies, respectively. The bars represent 400-m distances. These are representative data for three independent experiments.

Article Snippet: Cells were washed three times with staining buffer and incubated with donkey anti-mouse (CD81) or anti-rabbit (SR-BI) antibody conjugated to phycoerythrin (Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:100 dilution for 30 min at 4°C.

Techniques: Expressing, Infection, Incubation

Journal: Journal of Virology

Article Title: Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I

doi: 10.1128/jvi.01134-06

Figure Lengend Snippet: FIG. 3. MCD treatment of Huh-7 cells decreases CD81 plasma membrane expression. CD81 expression was analyzed in mock-treated Huh-7 cells (A) or in Huh-7 cells after treatment with 10 mM MCD (B). CD81 expression was also determined in MCD-treated cells to which exogenous cholesterol was added (C). SR-BI expression was also analyzed in mock-treated (D) and MCD-treated (E) Huh-7 cells. The bars represent 60-m distances. These are representative data for at least two independent experiments.

Article Snippet: Cells were washed three times with staining buffer and incubated with donkey anti-mouse (CD81) or anti-rabbit (SR-BI) antibody conjugated to phycoerythrin (Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:100 dilution for 30 min at 4°C.

Techniques: Clinical Proteomics, Membrane, Expressing

Journal: Journal of Virology

Article Title: Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I

doi: 10.1128/jvi.01134-06

Figure Lengend Snippet: FIG. 4. CD81 cell surface expression decreases after MCD treatment. Cell surface expression of CD81 (A) and SR-BI (B) was analyzed in mock-treated Huh-7 cells (M), Huh-7 cells that were treated with 10 mM MCD (MCD), or MCD-treated cells which were further incubated with cholesterol (MCDC). (C) MFI of CD81 surface expression after mock treatment or treatment with 10 mM MCD in the presence (MCDC) or absence (MCD) of exogenous cholesterol are plotted. These are representative data for at least two independent experiments.

Article Snippet: Cells were washed three times with staining buffer and incubated with donkey anti-mouse (CD81) or anti-rabbit (SR-BI) antibody conjugated to phycoerythrin (Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:100 dilution for 30 min at 4°C.

Techniques: Expressing, Incubation